OBJECTIVES
1. Define and understand the history of biotechnology and genetic engineering.*
2. Have a basic understanding of plasmid DNA and how it differs from chromosomal DNA.*
3. Explain how plasmids are used for cloning genes of interest. *
4. Understand the steps involved in the cloning of genes in bacteria.*
5. Describe why bacterial DNA replication/cell division is important in gene cloning.*
6. Describe the steps of gene expression (transcription and translation, Chapter 17) and how it relates to the manufacturing of pharmaceuticals from recombinant bacteria.*
7. Give examples of pharmaceutical products that are produced in recombinant bacteria.*
8. Describe the advantages of manufacturing pharmaceuticals in recombinant bacteria.*
9. Give examples of products that can be produced by transferring foreign genes into other microorganisms and plants.*
10. Define gene therapy and list the diseases that are now being used as models for gene therapy.*
 

KEY TERMS
Biotechnology, genetic engineering, plasmid DNA, gene cloning, gene expression, gene transfer.